why we work always in the dark when using fluorochromes?
because there is possibility of quenching of that particular dye at a particular wavelength of light that why always prefer dark.
Hi, I have a Gaussian cube file (.cube) that I obtained with gmx spatial. It represents the spatial density of cholesterol during a simulation (Protein in a membrane with explicit solvent). I...
03 April 2019 9,209 1 View
Hi, I have a Coarse-grained simulation of a dimer with 5 transmembrane helices for each monomer and I would like to calculate the tilt angle of each monomer with the respect to the membrane...
31 December 2018 6,042 2 View
Dears, I run several Coarse grained simulations of a transmembrane protein using Martini FF. In order to do a deep analysis of my systems, I backmapped a part of the trajectory from CG to AA....
09 October 2018 4,416 2 View
Dears, I run MD simulation on a transmembrane protein. When I visualised the frames, it seemed that I have some loss in alpha-helix (TM region). How can I quantify this? NB: I used timeline of...
07 August 2018 4,220 4 View
Dear all, I would like to simulate a protein which is located in the Mitochondrial Outer Membrane (OMM). I was searching in the literature about lipid composition of this latter (in Mammals) but...
01 February 2018 5,091 2 View
Dear all, I'm running simulations on membrane proteins (POPC) using Desmond software and I would like to use GROMACS tools to analyze certain parameters related to the membrane. Exemple: -...
02 March 2017 1,072 2 View
Dear all, I run MD simulations using GROMACS of a protein in the apo and holo form and I have to analyse the differences between the two forms. What kind of tests that can be performed? Thank...
10 November 2016 8,182 5 View
Dear all, I'm working on simulating a homodimer with its cofactors (hem, THG) and its substrate with GROMACS. Since my system is complex, I decided to start first with a monomere taking only...
04 May 2016 706 2 View
Hello, I have missing atoms and residues in my PDB file and I need to fix them in order to proceed with pdb2gmx (GROMACS). I used swissModel but the generating file is lacking the final residue in...
04 May 2016 7,559 6 View
Hi every one, i need ur help please: i'm preparing my final project about the implication of autoantobodies and inflammatory proteins in systemic sclerosis and i can't find any articles about...
04 May 2011 8,324 3 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches
04 August 2024 2,703 6 View
Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...
31 July 2024 8,141 13 View
I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...
30 July 2024 4,267 2 View
Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...
29 July 2024 7,198 9 View
Employing a pragmatic inquiry research design, looking for published research using this method, employing qualitative research data collection methods of semi-structured interview and focus...
28 July 2024 540 2 View
First, thanks for taking the time to read my puzzle 🥹! I am currently working on the methodology part of my dissertation, and I have chosen a questionnaire as the instrument for my quantitative...
27 July 2024 3,457 3 View
I know the difference between instrumental LOD and method LOD but my query is - in case of any sample whose concentration is zero or not detected by the instrumental LOD, is it possible to get...
24 July 2024 6,592 5 View
Are both ways acceptable, or is there any convention to them? Thanks.
22 July 2024 6,988 4 View
Greetings! I am looking for materials on quantitative and qualitative research. What are the methods of collecting information and the principles of their implementation? I would like to make an...
20 July 2024 2,033 7 View