Its a novel technique to manage the insect pest. RNA interference (RNAi) is a gene silencing mechanism triggered by providing double-stranded RNA (dsRNA), that when ingested into insects can lead to death or affect the viability of the target pest. Transgenic plants expressing dsRNA version of insect specific target genes are the new generation of resistant plants. Syed Arif Hussain Rizvi
RNAi can paly an important role in gene silencing technology as a main tactic of IPM of insect pests. When OR protein heterodimerized with olfactory co-receptor (Orco), it plays an important role in selectivity and sensitivity. Deleting Orco has been resulted in degrading OR protein function, and therefore, Orco silencing may inhibit the response of insects to the pheromones, which could provide a novel tactic for management of insect pests. A molecular method using olfaction interference has the potential to disrupt insect mating and plant locating, and thus disturbing the insect reproduction. Orco disruption by silencing the gene (RNA interference, RNAi) is used widely in protecting crops. The olfactory detection genes were identified in some insect pests. Injection insect by dsRNA resulted in a significant diminishment of RferOrco expression as compared to control insects. It seems that olfactory disruption applications could successfully lead to the development of a novel pest-management strategy for eradicating insect pests.
The RNA interference method consists in the destruction of double-stranded RNA, which is not synthesized in the plant cell, but is somehow found there. This RNA is inherent in a foreign nuclear organism, which completely destroys the plant in a short time. If we take this potentially viral RNA and cut it with the help of special proteins into very small pieces, which will later act as a reference model in the search for similar mRNA, the cell's own genes of a malicious object. According to the essence of RNA interference, if suddenly the sequences of the cut parts coincide with the cell's own genes, and at the same time they are vital, then the RNA of such cells will be destroyed, and the cells themselves will die.
GreenLight is developing seven products that it believes will be approved for use by 2026.
RNA interference (RNAi) is a gene silencing mechanism triggered by providing double-stranded RNA (dsRNA), that when ingested into insects can lead to death or affect the viability of the target pest. Transgenic plants expressing dsRNA version of insect specific target genes are the new generation of resistant plants.
https://www.intechopen.com › books
Management of Insect Pest by RNAi — A New Tool for Crop Protection
Silencing of some genes had produced devastating effects on the insect growth, development, and survival (Xu et al. 2016). ... In plant-mediated or host-induced RNAi (HI-RNAi) approach, a crop plant is engineered with hair-pin RNAi vector to produce dsRNA against the target gene of insect pest.Apr 29, 2017
https://www.ncbi.nlm.nih.gov › pmc
RNAi technology: a new platform for crop pest control - NCBI
In insects, the siRNA pathway is activated when double-stranded (ds)RNA molecules, as products of viral replication, are recognized in the cytoplasm and processed into siRNAs of 18-24 nt by the RNase type III enzyme Dicer-2 (Siomi and Siomi, 2009).Jan 11, 2019
https://www.frontiersin.org › full
RNA Interference in Insects: Protecting Beneficials and Controlling Pests