In testing recycled water, as derived from municipal wastewater treatment plants, when testing using the coliform multi-tube most probable number (MPN), the results are typically low counts to non-detect. But when one moves down the pipe to where that same water is being used and runs a second series of tests (MPN), the numbers are often so high to be off the charts. Thus, was the initial test at the plant a false negative? Was there some kind of resuscitation of bacteria from a viable but non-culturable state, a state that was not picked up and could not be picked up by the initial testing? How do these results affect the durability of this water as a sound source for irrigation of crops consumed raw?
Dr Edo McGowan