I like to extract into methanol, followed by ion exchange. You'll still have a lot of phenolic compounds.

(PDF) Purification of phenolic flavanoids with flash chromatography (researchgate.net)

you can do the maceration extraction method by using ethanol 70% or 96% for 24 hour and then it can be evaporated by using rotary evaporator instrument. so you can collect the extract.

by using the reference chemical compound (eg. quersetin) and setting the HPLC condition you can check the consentration the specific chemical compound (eg. quercetin) by AUC result.

if you do fraction part (non polar; semi polar; polar) you know the compound part of solvent type.

for the next part, you can do purification by isolation the specific compound of herb.

thank's

Thank you. If I have a single phase mobile HPLC device

How can deal with this situation in the diagnosis of effective substances