What is the Timestep definition in the Conservation Element / Solution Element in 3D ?

15 November 2020 1,214 3 View

Is free cytosolic GFP fluorescence excluded or reduced in the endomembrane system (ER, Golgi, lysosomes)? Why or why not?

As soluble, untagged GFP is not membrane bound and doesn't contain targeting or signal sequences, one might expect it to be synthesized by free ribosomes and not bound ribosomes. That would mean...

08 March 2020 997 3 View

How many base pairs mismatches should i consider to design a specific primer? my templates have 1-2base pairs mismatch only when i aligned them?

i need to design a specific primer for each of my samples. but my samples, when i aligned them, they only have 1-2 bp mismatches, barely 3

17 September 2019 6,650 3 View

Can you recommend a fluorescent ELISA agent?

Using a fluorophore rather than a chromophore for ELISA detection seems like an obvious solution to increasing ELISA sensitivity, but I'm struggling to find work done in this area. Can anyone...

07 August 2019 2,604 5 View

What are the Arch Garch conditions?

20 April 2019 3,704 4 View

What is a best way to immobilize RGD peptide with electrospun nanofiber?

I synthesized the nanofiber via electrospinning but need to find a best way to immobilize RGD peptide (Arg-Gly-Asp) onto naofibers.

03 September 2018 8,448 1 View

How to run longitudinal mediation with multiple (5 waves)?

Hi everyone! I'm doing a PhD in Clinical Psychology and I have some treatment data to analyse. The design is a 2 (condition: self-compassion, cognitive restructuring) x 5 (time: baseline,...

01 August 2018 4,502 3 View

Increased thermopower (Seebeck coefficient) with rising temperature? (p-type semiconductor). Is this the case for any p-type sc? What about n-type?

28 March 2018 4,475 3 View

Why do my western blots look so odd?

The three images attached here are for 3 different proteins probed at the same time. The first one is GAPDH. Does anyone have any idea why (1) Despite reasonable Bradford assay results, GAPDH...

14 January 2018 5,544 3 View

Are plasma protein free cysteines truly free?

free as in unreacted sulfydryls, not free as in speech, nor free as in beer :) I am working with an 80 kDa plasma protein with 5 cysteines, 2 of which are in a disulfide bridge. There are a lot...

14 December 2017 7,799 13 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Is there an open source free software for text mining and sentiment analysis?

I am looking for a free software for text mining and sentiment analysis for my research on customer review mining (it involves calculating polarity of attributes,opinion oriented information...

25 February 2021 1,920 6 View

Suggest me some trending topics for phd research in text mining/AI/machine learning?

My master's research is in info retrieval and text mining. I would be grateful if you could help me to select a good topic for my phd research proposal.

24 February 2021 393 14 View

What is/are the best ML classifier(s) for Sentinel2 images?

Hi I am trying to segment a sentinel2 image. At this stage, I want to run a binary classifier that assigns each pixel to either farm or non-farm pixel. For this purpose, I have 4 10m bands...

15 February 2021 3,596 9 View

Can you remove a publication from me?

I thought it might be mien from decades ago when I did research in math, but it is not mine.

03 February 2021 7,712 4 View

What is the best geophysical machine or device for detecting groundwater?

what is the best geophysical machine or device for detecting groundwater? Are there any machine that can detect the depth and the type of water? do I have to buy one machine or more than one to...

26 January 2021 6,149 15 View

Is Handheld XRF analyzer useable? and accurate in geochemical test?

What is the different between Lab XRF and Hanheld XRF, is it accurate and useable to analyze sedimentary rocks?

23 January 2021 9,941 6 View

Do you have information about synthetic diamonds and their environmental impact?

I am doing a deep research on the comparison of the process of extraction of natural diamonds and synthetic diamonds, and their environmental impact I would appreciate you could share your...

19 January 2021 1,359 3 View

What's the correct way of treating ordinal variable as continuous in regression?

08 January 2021 920 16 View