Hello everybody,

I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish.

I have three treatments (control, low dose, high dose) in my experiment and 4 replicates (n=4). So a total of 12 tanks. Now, the fish from one tank are counting as dependent samples and therefore actually pseudo-replicates. Does it still make sense to sample more fish per tank? Do I have to pool the resulting data afterwards, as I statistically wouldnt be "allowed" to evaluate the fish from one tank on a per-fish basis? If we now assume that I sample 3 fish per tank, i.e. 12 per treatment (3x 4 replicates) and I have no significant tank effects, could I then increase my "n" to 12?



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