Seem that a lack of common standards about studies of Nanoparticles cytotoxicity and others nanomateriais for interaction with living cells has been one of the limitations of the current research, mainly in neurosciences, which invoves diifferent cell lines, exposure times, and colorimetric assays that usec in different studies. For this reason, I think that it is necessary we beginning a discussion about this theme so that will be possible to compare cytotoxic effect among these results, such as single standard meets all conditions for obtaining information on nanomaterials cytotoxicity. What do you say about this theme?
Hi! It is a good idea to start discussion since mechanisms of toxocoty of nanostructurescpuld vary from one material to other. One ofproblem could be high heterogenity of nanostructures pool (different size of particles, for example).
Try for starters:
ASTM E2526 - 08(2013) Standard Test Method for Evaluation of Cytotoxicity of Nanoparticulate Materials in Porcine Kidney Cells and Human Hepatocarcinoma Cells
ASTM F895 - 11(2016) Standard Test Method for Agar Diffusion Cell Culture Screening for Cytotoxicity
ASTM F813 - 07(2012) Standard Practice for Direct Contact Cell Culture Evaluation of Materials for Medical Devices
Also try: ASTM STP810 Cytotoxicity Testing: Prediction of In Vivo Toxicity from In Vitro Tests Published: Jan 1983 http://www.astm.org/DIGITAL_LIBRARY/STP/PAGES/STP30156S.htm
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