How to remove compound ( Europium complex ) from paraffin oil?

How can I remove my compound from paraffin oil? Mistakenly my compound fell in an oil. Compound = Europium complex

22 February 2021 5,667 2 View

Regarding Chemkin.. no reaction taking place after simulation. What to do?

I am simulating cyclohexanone oxidation from a paper. I got the input files from supplementary data. But when I am simulating, and generating Excel file at different temperatures the reactant...

02 October 2020 6,591 4 View

Can someone provide the list of cell lines available at NCCS, Pune, India?

I need to purchase a few cell lines but I am not sure whether those are available with them or not.

31 December 2018 3,313 7 View

Can someone suggest some brain endothelial cell lines?

Preferably Biosafety level 1. Also, if you can tell from where we can procure them in India.

11 December 2018 8,356 6 View

Can someone suggest a method for revival of THP-1 cell line?

Cells are healthy when used routinely but once freeze downs are made (FBS + 10%DMSO) and stored in -80 C their revival is quite difficult and majority cells are dead. I am using RPMI-1640 media...

15 June 2018 5,833 3 View

Is there a standard method for extraction of essential oil of aromatic plant leaves using solar distillation technique?

28 September 2017 4,608 3 View

PCR amplification of nearly 200bp fragment from bisulfite converted DNA not working.

I am trying to amplify nearly 200bp segment of DNA. Changed primer concentrations, template concentration, primer stock everything, still no amplification. Seeking help.

17 July 2016 1,417 6 View

How can one ensure ligation of a 300bp fragment into 5369bp host?

18 October 2014 5,087 15 View

How to use a Western Blotting specific antibody for IHC (for PFA fixed & paraffin Embedded tissue)?

I have an antibody in my lab specefic for western blotting only, but I need to detect same antigen in a PFA fixed tissue. I tried to retrieve epitopes using Citrate buffer, HCL treatment &...

16 October 2014 5,595 7 View

Can anyone comment with examples on the idea that disasters set the development clock back to several decades?

Recently, the increasing number of disasters has shook the world. Be it the cloud burst in Uttrakhand, India or cyclone Katrina in USA or Typhoon Haiyan in Phillipins. What is the exact amount of...

30 November 2013 4,686 4 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View