In what are you trying to measure the ethanol? For example, if you are trying to determine the amount ethanol in automobile fuel (oxygenated gasoline), there is a physical way to do so: in a graduated cylinder with a ground glass stopper, place a known volume of fuel, add a known volume of water, and then replace the stopper and shake. The dividing line between the hydrocarbons and the water will change depending on the amount of ethanol in the fuel.
Dear Yerra Kanakaraju thank you for posting this interesting technical question on RG. In order to give you a qualified answer, it would be helpful to know a few more details about the conditions / environment in which you want to determine the ethanol content (e.g. in blood, alcoholic beverages, biodiesel etc.). In general, NMR spectroscopy is a suitable method for determining ethanol in mixtures. For some potentially useful information please have a look at the following article which might help you in your analysis:
Determination of Alcohol Content in Alcoholic Beverages Using 45 MHz Benchtop NMR Spectrometer
Article Determination of Alcohol Content in Alcoholic Beverages Usin...
This method does not require fancy and expensive equipment. The good thing about this paper is that it is freely accessible as public full text on RG. Thus you can download it as pdf file.
I would like to explain the estimation of ethanol as it's applications in gasoline technology as biofuel. The researchers used ethanol as oxygenayed gasoline additives. We can estimate the amount of ethanol in motor gasoline using Gas chromatography in order to know the hydrocarbon compositions. From the GC, we can check the oxygen content in whole gasoline sample as reformulated gasoline. If the finished gasoline sample contains oxygen. We will see the components, which related ethanol.