Hello everyone,

I would like to use Rstudio to analyze TCGA data and generate a heatmap using the heatmap package. I try to divide the patient sample into two groups according to one gene expression and try to analyze other gene expression differences between these two groups. But after doing pheatmap it shows: Error in check.length("fill") : 'gpar' element 'fill' must not be length 0.

Then I check the annotation_col and it shows like this.

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