Tianyi Wang

5 Questions 7 Answers 0 Followers

Questions related from Tianyi Wang

Error in check.length("fill") : 'gpar' element 'fill' must not be length 0?

Hello everyone, I would like to use Rstudio to analyze TCGA data and generate a heatmap using the heatmap package. I try to divide the patient sample into two groups according to one gene...

18 December 2021 8,927 3 View

About stable transduction of FUCCI plasmid in the cells?

Dear all, I got these two plasmids from Addgene (#86849 and #138714) and tried to transduce into MDA-MB-231 cell line using the lentivirus. Therefore, I packaged these two plasmids in 293T cells...

06 September 2021 8,729 6 View

How to get conditioned medium from Raw264.7?

Dear all, I would like to see the effect of my drug on Raw264.7 polarization and I would like to use the Raw264.7 conditioned medium to treated cancer cells to see the anti-cancer effect. And I...

07 November 2020 4,663 2 View

Why use dextran-conjugated fluorescent dye for tracking macropinocytosis?

I have read some publications and it seems like dextran-conjugated fluorescent dye is a perfect and specific macropinocytosis marker. And based on this article (Li, L., Wan, T., Wan, M., Liu, B.,...

05 February 2020 3,731 0 View

Why starvation for 4 h leads to my MCF7 cell death significantly?

I am currently doing some work about starvation-induced autophagy. I treated the cells with EBSS medium without FBS for 4 h to induce autophagy. The morphology of the cells got strange. And when...

06 December 2019 1,385 4 View