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Questions related from Tianyi Wang
Hello everyone, I would like to use Rstudio to analyze TCGA data and generate a heatmap using the heatmap package. I try to divide the patient sample into two groups according to one gene...
18 December 2021 8,980 3 View
Dear all, I got these two plasmids from Addgene (#86849 and #138714) and tried to transduce into MDA-MB-231 cell line using the lentivirus. Therefore, I packaged these two plasmids in 293T cells...
06 September 2021 8,748 6 View
Dear All, I would like to use some mTOR inhibitors like OSI-027 to do the cellular thermal shift assay (CETSA) to see the interaction between this molecule and mTOR protein. However, I did not...
01 December 2020 9,002 0 View
Dear all, I would like to see the effect of my drug on Raw264.7 polarization and I would like to use the Raw264.7 conditioned medium to treated cancer cells to see the anti-cancer effect. And I...
07 November 2020 4,680 2 View
I have read some publications and it seems like dextran-conjugated fluorescent dye is a perfect and specific macropinocytosis marker. And based on this article (Li, L., Wan, T., Wan, M., Liu, B.,...
05 February 2020 3,750 0 View
I am currently doing some work about starvation-induced autophagy. I treated the cells with EBSS medium without FBS for 4 h to induce autophagy. The morphology of the cells got strange. And when...
06 December 2019 1,410 4 View
Some publications use microglia cells such as BV2 to investigate its role in autophagy and Amyloid beta clearance, while others use neuroblastoma cell line like N2a and SH-SY5Y. I wonder if I...
29 May 2019 5,687 3 View
