Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Shall we split the data into 60% of training and 40% of testing. And apply k fold cross validation for training and the best trained model for test?

Is the above method of splitting the data set into taring and testing, and then apply k fold cross validation for training, and then the trained model to classify the test set is right?

04 April 2020 9,040 3 View

What would libraries do to stop students suicidal act on campuses?

In recent times, students have been involved in committing suicide. I think the academic libraries would play some roles in stopping this act

12 November 2019 7,449 3 View

Scalogram for huge data?

I have a huge data for which I want to apply wavelet and plot scalogram. the below code works for small data. However, I have a huge signal, for which I am not able to plot scalogram. Can anyone...

10 April 2019 1,959 3 View

How to do miRNA isolation from hematopoietic stem cell with good purity?

Hi, currently I am isolating miRNA from hematopoietic stem cell using miRNeasy Qiagen kit. However, I didnt get good purity for both A260/280 and A260/230 plus low concentration as well. According...

14 October 2018 6,123 1 View

What are the causes for low Free Fatty Acids (0.16) in Cocoa mass?

Free fatty acids content should not be more than 1.75% in cocoa mass according to FSSAI standards and got 0.16 % as result. I want to know the reason behind occurrence of low free fatty acids in...

22 November 2017 9,993 4 View

In thin films, how resistivity concentration and mobility of charge carrier are inter related?

In thin films, how resistivity concentration and mobility of charge carrier are inter related?

09 November 2017 4,331 3 View

How to calculate refractive index of Zno thin film from UV data?

10 October 2016 135 3 View

How to concentrate DNA? I lost 70% of DNA while concentrating it using 3M sodium acetate and 2 volumes of ethanol? Is there any other alternative?

Read 7 answers by scientists to the question asked by Ramya Murugesan on Oct 6, 2016

05 October 2016 9,702 6 View

Is fluoxetine stable in chlorinated water (2ppm) for chronic per os treatment?

Our animal facility has chlorinated water (2ppm), and we would like to administer chronic fluoxetine in the drinking water

27 August 2016 9,683 2 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View

Please suggest the website or link for the collection of data for (Cancer Incidance Rates in India)?

We are doing research for the prediction of cancer incidance rates increases in India upto next five years. Please help us to get the exact data for Cancer Incidance Rates in India. This will...

19 February 2021 8,478 2 View

Access to supercomputer resources in India?

Hello everyone, I want to perform some gaussian calculations and want to use Materials studio for my research work. Can anyone guide me how to get access to supercomputer resources in India for...

15 February 2021 8,264 3 View

Extraction of DNA from insect samples conserved in silica gel?

Hi everybody, can anybody suggest how to extract DNA from insect samples conserved in silica gel (in my case, mosquitoes)? Does anybody know a commericial kit which does that or a particular...

10 February 2021 3,392 4 View

What is India's InternationalTrade Policy during Pendamic? How do you find the policy as successful one?

During Covid-19 Pendamic Government are facing problem to maintain business. India is one of them so this question is perfect to be discussed here.

08 February 2021 2,725 3 View

Which satellite image gives best results for Land Use land cover classification(specifically for built up regions) for Indian region ?

Landsat, sentinel and USGS satellite images have varying accuracy for identification of urban built up dynamics ? Which one is best suited for India

04 February 2021 8,632 8 View

MAD or boxplot to detect outliers in non normally distributed data for latency and distance in animal cognition?

I am studying animal cognition and I have data about animals' latency to approach a cue and animals' distance from the cue in a test. Data are not normally distributed and when I do scatterplots...

04 February 2021 503 3 View

What is the role of IQAC to improve the grading of an institution?

IQAC provides all information about institution and its various activities to NAAC, Bangluru, India in order to get good grading. In this light what is the role of IQAC??

03 February 2021 6,916 1 View

I want some expert (free) advice and guidance in interpreting depositional environment from well logs in a deltaic setting. Anyone ready to help?

I am looking into the geophysical well logs from a coaliferous basin in India. I need some help in depicting the depositional environment from well logs.

02 February 2021 6,052 3 View