Whiçh will elite first in chloride, chlorite, chlorate, and perchlorate ions in ion exchange. If we go through molecular size the pattern of elution will be same as i mentioned above in the question itself. But when we are doing experiment, it was observed that chlorite is eluting first before chloride even though the molecular weight of chlorite is more than chloride. Kindly clarify?
What is the name of your column? Ion chromatography does not separate on the basis of hydrated radius alone. In chromatography, there are always multiple interactions which cause the anion to retard on the column. A classic case is that of bromide and nitrate. Which one has a larger molecular weight? Still nitrate is retained more on ion-exchanger columns. Google the concept of anion polarizability and ion chromatography retention.
What is the name of your column? Ion chromatography does not separate on the basis of hydrated radius alone. In chromatography, there are always multiple interactions which cause the anion to retard on the column. A classic case is that of bromide and nitrate. Which one has a larger molecular weight? Still nitrate is retained more on ion-exchanger columns. Google the concept of anion polarizability and ion chromatography retention.
See attached link, it's the US EPA method and shows chromatograms of elution patterns of the anions on different columns (last page). Also column manufacturers are a great source of information..for instance if you have a column from Dionex - see atttached IC column manual.
https://www.epa.gov/sites/production/files/2015-08/documents/method_300-0_rev_2-1_1993.pdf
https://tools.thermofisher.com/content/sfs/manuals/4445-Man-034791-12-IonPac-AS11-Apr10.pdf
- Badges
- Science method
- Similar topics
- Chemistry
- General Biochemistry
- Biochemical Techniques
- Analytical Chemistry