Dear all, Happy New Year!

Laser 505nm is not @Max abs peaks of Fam, Tet, and Hex, Hex as donor seems can cover acceptors with much longer wavelength, though Hex has lower absorption@505nm than Fam. Is it better to use Hex than to use Fam for acceptors with longer wavelength (FRET)? Will Hex's 505nm excitation efficiency be lower than that of Fam's or we can adjust the 505nm laser intensity to make up the absorption difference?

May be it is a silly question, do you think Hex will be a better donor than Fam for using 505nm laser, for acceptors with longer wavelength ?

Thank you very much!