Which is the viscosity recommended for using Methyl cellulose to perform oral gavage (mice )?

Hello, we would like to perform oral gavage in mice. The vehicle recommended for our drug consist on 0.5% methylcellulose and 5% Dimethylacetamide (0.1 ml/ 10g mouse). Could somebody help us to...

20 August 2020 8,720 2 View

Soft agar colony assay: Where should I put my treatment ?

I am performing a soft agar colony assay using 2 different cell lines. Both are able to make colonies. So now I want to test a specific drug and see if it can affect their capability for making...

15 December 2016 4,656 3 View

How to breed whiteflies (Bemisia tabaci)?

I need to establish a breeding for my project. Can anybody suggest a suitable book/paper dealing with this issue?

25 November 2013 885 5 View

How could we prevent dimerization of thienopyrimidinone in basic conditions?

I'm working on nucleophilic substitutions on 2-(chloromethyl)thieno[2,3-d]pyrimidin-4(3H)-one with phenol/thiophenol/aniline derivatives (such as di phenylureas or diphenylacetamide). I have tried...

10 October 2013 502 14 View

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More and more often, countries all over the world are fostering research. What can be done so that all researchers understand each other better? How can this help researchers work cooperatively...

26 February 2013 6,238 13 View

Which will be a good machine learning technique for implementing text categorization?

By categorization i mean assigning text to a particular class

25 February 2013 4,173 9 View

I am working on text categorization using machine learning techniques. Is there any reliable input encoding scheme for the same?

The categorization is for a sentence/word in to a particular class

25 February 2013 857 1 View

Could I research with your skills, experiences?

I built a frame on Topological quantum modular computers control system.

01 January 1970 3,365 1 View

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In my experience in Programas applcated to learning is very effective the realtion between hearing ang language... Thank you very much. Pilar

01 January 1970 1,151 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

What are the potential shell well and atomic solvation parameters for the boron atom?

I have a protein-ligand docking project. The ligand contains boron atoms that are not detected by Autodock software. To solve this problem, it is necessary to introduce the boron atom to...

19 February 2021 1,236 1 View

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17 February 2021 4,217 3 View

How to perform Kaplan-meier survival analysis in SPSS?

Hi everyone, This is my first time of attempting to run survival analysis. I have a data on patients with End-stage Kidney Diseases (ESKD). I would want to run survival probability by treatment...

16 February 2021 2,647 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

What is the best substrate for exfoliating few layers of hexagonal boron nitride (hBN) using traditional scotch tape method?

Unlike graphene, it is usually very difficult to exfoliate few layers (~10 nm thick) of hBN on SiO2 substrate due to lip-lip interaction at the edges. Au substrates have been proven very useful...

16 February 2021 2,635 2 View