I am trying to recreate a method I found for DNA extraction from soil samples which used TEN buffer. The concentration for TEN were as follows: 100 mM Tris-HCl, 100 mM EDTA, 100 mM sodium phosphate, and 1.5 M NaCl. I am wondering if I would be able to replace the sodium phosphate with potassium phosphate, since there is potassium phosphate within the lab currently but no sodium phosphate. I can't think of a reason that potassium being in solution in place of sodium would be problematic, but I am also not the best chemist. If I did try to replace sodium phosphate with potassium phosphate, should I also replace NaCl with KCl?
If you replace sodium phosphate with potassium phosphate, I don't thing that you should also replace NaCl with KCl; because Both NaCl and KCl play the same role in this buffer: they Increase the ionoc strength of the buffer; thus if NaCl or KCl is added in the buffer at the same concentration, the ionic strength will be near the same.
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