FRAP is an antioxidant test that measures the reducing activity of an extract. it can be expressed in mgEstandard/100 g. with different concentrations how do you calculate EC50?
Saif Wahid
Prepare a series of concentrations of the extract or compound you are testing. These concentrations should cover a wide range, from low to high. Perform the FRAP assay for each concentration of the extract. Measure the reducing activity of the extract at each concentration. Typically, the reducing activity is expressed in mgEstandard/100 g. Plot a graph with the concentrations of the extract on the x-axis and the measured reducing activities (mgEstandard/100 g) on the y-axis. Determine the concentration at which the reducing activity is half of the maximal activity. This can be estimated by visual inspection of the graph or by using a statistical method such as nonlinear regression analysis. The concentration at which the reducing activity is half of the maximal activity is the EC50 value. It represents the effective concentration at which the extract exhibits 50% of its maximum reducing activity.
To calculate the EC50 (half maximal effective concentration) using different concentrations in an FRAP (Ferric Reducing Antioxidant Power) assay, you can follow these steps:
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