Hi Xun-Wen,

You have to use an inducible vector system for that. When you want RNAi expression, you add a metabolite or other molecule to induce your vector system to produce RNAi of interest. When you take out all of the metabolite from the culture, RNAi production just stops. You can check protein levels to show that.

Good luck

Hi Cigdem, Thank you for the reply. It gives me some hints that i can dig further.