Dear researchers, Hi;
I want to know the frequency sweep( temperature equilibrium time)
Hi, I've N2 gas cylinder and 100 ppm NH3 gas cylinder. With these two cylinders, I'd like to make a ppb level (1- 100) concentration. Any idea how to do that?
01 February 2021 3,264 2 View
Whether L version and D version peptides should have identical NMR, especially in the state of self-assembly have to have identical NMR? How to use HRMS to confirm L version or D version...
01 February 2021 6,756 4 View
Hi, I am doing RNA extraction for my final year project. After extraction of RNA by using conventional method, I did quantification and qualification of RNA by using Nanodrop spec and gel...
11 January 2021 8,775 10 View
Hey guys, I always have problems with the attachment of HEK293t cells to glass coverslips while doing IFA. They are so easy to detach from the coverslips while washing. The coverslips are already...
30 December 2020 8,381 3 View
Animals were fed DSS via drinking water and fecal samples were collected and DNA was isolated using Qiagen stool DNA isolation kit. The V4 region of extracted DNA was amplified with specific...
20 December 2020 1,739 3 View
Hi, everyone: I want to determine whether IL-2 and IL-15 have different effects on immunized CD8+ T cell effector function. In this study, I immunize WT mice with peptide on day 0 and 7, and...
02 December 2020 9,413 3 View
Respected researchers, I conducted a SEM including 5 variables (two independent variables , two mediators, and a dependent variables), the correlations between these variables range vary from...
01 December 2020 1,338 5 View
Does anyone know any glass slides (thickness 0.1 to 1mm) that have very low autofluoresence (GFP). I am doing a very sensitive experiment that require my glass substrate to be as low fluoresence...
28 November 2020 1,917 6 View
In Northern blot, we normally use either 2*SSC+0.1% SDS or 0.1*SSC+0.1% SDS in washing step. Does anyone know what's the function of the SDS in the washing buffer? Thank you in advance
26 November 2020 2,432 1 View
Suppose an agricultural field with uniform soil texture and salinization. The groundwater depth is 20 meters, assuming that the depth of the groundwater and the concentration of salts remain...
25 November 2020 2,382 4 View
Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...
19 February 2021 472 5 View
All, I am trying to equilibrate an Ice 1h crystalline structure to 250K in LAMMPS. When I tried to do this at 250K directly, the structure lost it's crystalline shape and became disordered post...
14 February 2021 5,747 2 View
I have a box which is kept at 4C which is enclosed in a ziploc bag. The dimensions of the box are: 18cm x 13.4cm x 4.9cm. The relative humidity of this internal environment is 30% at this...
07 January 2021 6,734 3 View
Greetings, I am trying to run GROMACS 2020.3 on a supercomputer using this command to do the production run: mpirun -np 24 gmx_mpi mdrun -v -s AB_md_0_1.tpr -deffnm AB_md_0_1 >...
17 December 2020 1,232 3 View
According to below articles about kinetic modelling of ODH reactions by G. Che-Galicia and et al. there are some problems need your guidance and advices: Kinetic and reactor performance of a...
27 November 2020 9,035 3 View
I want to investigate the reaction between a mineral (Calcite) and a gas phase (H2) which has been stored at a depleted gas reservoir. I try to model this by defining a solution and put H2(g) as...
25 November 2020 8,916 1 View
We consider a reaction system "A" reversibly reacts with "B" through k1 and k-1 rate constant respectively, and "B" gives "C" and "D" through k2 and k3 rate constant respectively through parallel...
27 October 2020 8,987 16 View
Hi everyone, I am new gromacs user. I am doing the MD simulation of protein-NA. I did the Energy minimization step, NVT, NPT equilibration. after equilibrating the system i submitted the...
21 August 2020 7,876 4 View
Dear people, I have optimized a dimer (each monomer contains about 50 to 60 atoms) structure in its equilibrium distances (I have done a rigid scan to find the equilibrium distance between...
05 August 2020 7,683 1 View
In most mathematical problems considering voids, why does the equation of balance of Equilibrated force not include any thermal relaxation parameter considering Green-Lindsay theory or any higher...
20 July 2020 5,481 6 View