During tubulin extraction, why is the cerebellum discarded and only cerebrum is used?

20 July 2013 0 2K Report

I read a protocol for tubulin extraction which took only the cerebrum and discarded the cerebellum and other parts of the brain. Why is that?

Badges
Science topic

Similar topics
Filing

More Sankalp Verma's questions See All

Can we mark 'EFL Learners shifting from general digital to AI technologies' as technological transition?

After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...

08 August 2024 8,964 4 View

How to generate a citation of my paper from ResearchGate?

How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...

08 August 2024 6,690 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to fix background error in rietveld refinement of one XRD peak using GSAS-II?

I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II

05 August 2024 5,291 2 View

How can I add own Henry coefficients in Aspen Plus?

Hi, i would like to simulate an absorption process in Aspen Plus. I want to use the NRTL model und would like to add some individual Henry coefficients. Is that possible and how?

05 August 2024 2,333 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

Can usage of AI tools like chat GPT in research work is recommendable ?

AI tools like ChatGPT can enhance research work significantly when used responsibly and in conjunction with thorough human oversight.

05 August 2024 1,842 3 View

Usage of internal standards in LC-MS/MS analysis?

Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...

05 August 2024 3,084 6 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

How effective is the Citi Bloc standard basket in enhancing the accuracy and comparability of international construction cost assessments?

Citi BLOC Standard Basket Definitions: A standardized unit representing a fixed basket of construction materials, labor, and equipment costs priced in various cities. Purpose: To create a common...

04 August 2024 8,997 1 View

Is it okay to choose the second or third hits rather than choosing the top hit in BLAST ?

I have been trying to identify some fungal samples using ITS primers and Beta tubulin primes. I got one species from ITS as the top hit but the same species name comes as the second or third hit...

22 March 2024 9,080 0 View

My western blot exposures are weak and taking a long time to develop why?

My transfer buffer Ph is 8.7 but should be a 8.3 is this causing my exposures to be off also my bands look smeared. i am trying to troubleshoot and figure out why my chemiluminescence western blot...

12 March 2024 7,433 1 View

How do I count the number of nerves in cryosections of the cornea?

I wanted to know how I could count the number of nerves in cryosections of the cornea after staining with Beta III tubulin. What software is available for automatic or manual counting?

01 November 2023 2,325 3 View

How to improve Nuclear Extract Kit (Active Motif) to avoid cytoplasmic contamination of the nuclear extract ?

Hello, I'm using the Nuclear Extract Kit Active Motif to do TransAM NFkB assay kit after I'm working with non adherent cells : B cells, more specifically mantle cell lymphoma cells I have a...

30 August 2023 7,227 1 View

How to cause hydrolysis of GTP bound to tubulin dimers without causing polymerization?

I am trying to get pure GDP-bound tubulin. Presumably, ordering purified tubulin from bovine or porcine brain will have a mixture of GDP and GTP bound tubulin. I really need a pure GDP-bound...

29 August 2023 8,315 0 View

I am trying to polymerise microtubules under TIRF but I am unable to see any dynamicity. What could be the possible reasons for no dynamicity?

The final concentration of the tubulins is 30 uM and GTP is 1 mM

05 July 2023 6,279 0 View

Cytosolic / Nuclear Fractioning Inconsistencies ?

Hello all, I have recently started working with cytosolic/nuclear fractioning of HEK293T cells. My first few attempts were unsuccessful, in that there seemed to be pretty much equal...

25 June 2023 9,996 0 View

Is it possible to do the double staining of anti-beta tubulin antibody and propidium iodide(PI) staining for the co-culture of PC12 and C6 cells?

I am doing the PC12-C6 co-culture system and would like to double stain the cells to observe the cell to cell interactions. I first used the mouse FITC-conjugated anti-beta III tubulin antibody,...

11 June 2023 2,391 7 View

Is it possible to do the double staining of anti-beta tubulin antibody and propidium iodide(PI) staining for the co-culture of PC12 and C6 cells?

I am doing the PC12-C6 co-culture system and would like to double stain the cells to observe the cell to cell interactions. I first used the mouse FITC-conjugated anti-beta III tubulin antibody,...

11 June 2023 6,689 1 View

Is it possible to do the double staining of anti-beta tubulin antibody and propidium iodide(PI) staining for the co-culture of PC12 and C6 cells?

I am doing the PC12-C6 co-culture system and would like to double stain the cells to observe the cell to cell interactions. I first used the mouse FITC-conjugated anti-beta III tubulin antibody,...

11 June 2023 3,606 0 View