Hi,
I'm preparing for studying the effect of some pharmaceutical excipient on P-glycoprotein function in intestinal brush border. Unfortunately, we had some technical problems during preparation of everted gut sacs. So, can we prepare gut sacs without eversion by placing the test solution inside the gut sacs? then samples were collected from the outside acceptor compartment?
Thanks .
Hi Khaled,
You can use gut sac without eversion, however eversion of gut sac helps in getting more volume in contact, because without eversion less volume of solution of drug/ excipients can be used, however in everted sac you can put it in a suitable container containing your desired amount of medium.
Hope you find this answer helpful...
yes definitely you can proceed without eversion. But volume of your product should be such that, it does not affect delicate brushing of sac. Because high volume may stretch the sac beyond the limit and affect your result adversely.
Thank you.
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