Hello,
I am doing a cytotoxicity test. I am using the CellTiter Glo 2.0 Assay, to measure ATP with Luminescence. I use the Cytation 5 to do my luminescence readings.
I have a lot of variability between my duplicates.
I have tried a lot of things to remove this variability, such as recalibrating my pipettes, making sure I am consistent in my pipetting action. I use a 96well plate with white walls and a clear bottom. I have read that the clear bottom could be the issue.
In one test, I measured the luminescence with the clear bottom and with the bottom covered with white tape. There was no difference with the variability of my duplicates.
Does someone have other suggestions?
https://www.researchgate.net/post/How_can_I_limit_the_Large_variation_of_dual_luciferase_assay_Promega_ratio_between_dublicate_samples
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