Optimum conditions for gel electrophoresis of DNA fragment that 3.3kb long?

I want to know what would be best to use for gel electrophoresis of a 3.3kb DNA fragment. I would like to perform a gel extraction and cloning with the fragment. What would be the best voltage...

06 December 2018 3,754 1 View

How can I measure the surface coverage of carbon based nanoparticles on ITO glass?

I am working on the nanopatterning of carbon island deposited on ITO glass substrate and I want to measure the surface area of the glass that is covered by the carbon nanoparticle (number of...

24 September 2017 2,142 3 View

How to optimize solubility of my heterologous protein overexpressed at 0.04mM of IPTG and 26C incubation ?

Good morning/afternoon I am over-expressing a bacterial protein (dimer) in BL21 E. coli, which it works even with 0.04mM of IPTG, incubated at 26C. However, when I want to check the solubility of...

20 August 2017 2,306 5 View

I need the summary of Mattew finkbeiner and his method?

Subliminal priming with nearly perfect performance in the ... Matthew Finkbeiner summary of it and method

17 February 2017 2,388 1 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Can anyone provide the Support vector regression code structure of LIBSVM MATLAB or python ?

Please, if Gaussian Parameter optimization is possible in this code structure

28 February 2021 278 3 View

How to modify Johnson cook equation in ABAQUS?

I wanted to add an extra parameter to the existing Johnson cook equation in the ABAQUS simulation. How to add those extra parameters. Ref: A modified Johnson-Cook material model with strain...

28 February 2021 4,841 3 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

No module named 'tensorflow.keras.backend.tensorflow_backend' ?

System Description Cuda 11.2 GeForce GTX 1080ti tensorflow version 2.4.1 keras version 2.2.0 python version 3.8 I was getting the below error when I try to run my code on the...

25 February 2021 6,673 2 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View