Doubt about anti-mouse IgG-CFL 488 sc-362259 specificity?

01 January 2020 1 884 Report

I am using the rabbit anti-mouse IgG-CFL 488 sc-362259 from Santa Cruz and because some problems we performed a test using the secondary antibody without the primary and we realized this antibody is able emit fluorescence in all the cells. Anyone else had this problem? Santa Cruz discontinued this antibody.

James Drummond

What cells are you using. If your cells have Fc receptors they may bind the rabbit anti-mouse. Also, what blocking are you using. How old is your rabbit anti-mouse, how has it been stored? Maybe try a new lot, or a different manufacturer for your secondary.

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