How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

ITS primers for fungal characterization?

For example, I need to characterize Rhizoctonia and Trichoderma simultaneously. In literature they are giving ITS1 &ITS3 for Trichoderma and ITS2 &ITS4 for Rhizoctonia. In our lab there is...

19 January 2021 4,157 3 View

Is there an alternative for the enzyme mixture Driselase (from Basidomycetes) for Fusarium protoplast generation?

Dear all, is somebody familiar with an alternative to the enzyme mixture Driselase (from Basidomycetes) for Fusarium fujikuroi and/ or Fusarium graminearum protoplast generation? Were are using...

16 June 2020 5,865 2 View

Is there a catalogue of Trichoderma isolates? How are these isolates given names?

Hello everyone, I've been trying to find out how certain isolates are given a name and where to find a list of established names. e.g. there's T. asperellum T54, T. spirale T4, T. hazarnium T40...

10 April 2020 3,862 3 View

White fluffy growth at the base of Soyabean stem?

Good day, May you please assist me with the following: What is the white fluffy growth on the base of the soybeans stem(right at the bottom on the ground). It is very common, and plants are...

01 February 2020 6,339 5 View

Is it appropriate to inoculate a crop with arbuscular mycorrhizae and Trichoderma at the same time? Is it better to add both inocula separately?

Endophytic Trichoderma species are rather aggresive in their colonization of a substrate; I understand that they do it faster and more effectively than mycorrhizal fungi (e.g. Glomus spp.). It...

10 November 2019 9,263 7 View

Except CTAB method what is the best kit to extract Trichoderma spp. DNA?

I'd been tried CTAB method for this fungi many times but it gave my heavy RNA and unclear DNA bands, I don't know what is the problem and its solution.. so what is the best used kit for extraction?

10 November 2019 8,892 4 View

Trichoderma - literature on development of conidiophores/branchlets?

A question for Trichoderma specialists - has any research been done on the morphogenesis of conidiophores in Trichoderma and how that might relate to mycoparasitic structures? What I've noticed is...

08 September 2019 4,471 5 View

I wish to formulate trichoderma spore in talqa powder and liquid form. any one please suggest me ?

i wish to formulate trichoderma spore in talqa powder and liquid form. any one please suggest me ?

04 August 2019 4,211 3 View

I am trying to isolate protoplast form Leptosphaeria biglobosa with no luck. Please help?

Hello there, I am trying to isolate protoplast from Leptosphaeria biglobosa for a transfection experiment I am planning to do but so far after incubating my mycelia for 6 hours in 10ml of...

30 July 2019 4,179 3 View

I am trying to isolate protoplast form Leptosphaeria biglobosa with no luck. Please help?

Hello there, I am trying to isolate protoplast from Leptosphaeria biglobosa for a transfection experiment I am planning to do but so far after incubating my mycelia for 6 hours in 10ml of...

06 July 2019 9,163 4 View