I am trying to look for a way to analyse profilin content in different tomato fruit tissues (peels, flesh and seeds). It seems the ideal choice is to run LS-MS/MS.
Our lab is working on DNA, not proteins so proper settings for protein analysis are not established. Due to aforementioned difficulties, we would like to request protein analysis to elsewhere.
Then, it seems we need a reference material for quantitation of our protein of interest.
There, I'm curious about the reference material.
I've found it's not easy to purchase tomato profilin.
But there are similar profilins commercially available.
Like a profilin from birch pollens.
The sequences of two profilins are about 85% identical.
Can I use birch profilin as a reference material when identifying tomato profilin by HPLC?
If that's not a proper choice...
then should we produce recombinant tomato profilin by E. coli transformation?
I have one more question!
When running HPLC, what is the minimum amount of the reference material?
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