The two plots in the attached figures illustrate spectra for copper-polyethylenimine (Cu-PEI) and copper-polyacrylic acid (Cu-PA2) complexes. In the spectrum for Cu+1.7 ppm PEI (first plot) there seems to be a characteristic peak at 263nm. Two studies suggest a characteristic peak at 275nm. Is there a reason for this deviation? The studies also mention a characteristic peak from 550nm-750nm in the visible region. In the second plot, I think there is a peak at 246 nm for spectra generated for Cu+B and Cu+11.8 ppm PA2. B is a solution that is expected to contain PA2. However, in both plots there seem to be no absorbance in the visible region. Is this due to low Cu/PEI/PA2 concentration?
It's very possibe, as far as Beer-Lambert law is obeyed, the concentration will definitely affect absorbance.
Based on beer-lambert equation, the concentration affect the absorbence, in the determined range which has been mentined in refrences,
For more information You can see link below
https://en.m.wikipedia.org/wiki/Beer%E2%80%93Lambert_law
At certain concentration range, absorbance of sample solutions obey Beer-Lambert law. That is to say that the higher the concentration the higher the absorbance within this "window". However, as the sample solution becomes so concentrated, the certainty with predicting the absorbance decreases and and becomes more random. So, the answer to your question depends on the concentration range you are working with. If the concentration range is that which obeys Beer-Lambert law then, absorbance increases with concentration. But outside this range you can't say for sure how absorbance will behave with concentration change.
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