Ahmed,
presence of gelling agent or its type has strong effect on success of plant growth and differentiation.
http://www.tandfonline.com/doi/pdf/10.1626/pps.2.138
https://www.researchgate.net/publication/229797241_Effects_of_agar_brand_and_concentration_on_the_tissue_culture_medium
Regards
Alex
Article Effects of agar brand and concentration on the tissue culture medium
Ahmed,
presence of gelling agent or its type has strong effect on success of plant growth and differentiation.
http://www.tandfonline.com/doi/pdf/10.1626/pps.2.138
https://www.researchgate.net/publication/229797241_Effects_of_agar_brand_and_concentration_on_the_tissue_culture_medium
Regards
Alex
Article Effects of agar brand and concentration on the tissue culture medium
Dear Dr. Ahmed
Gelling agents type is important for the generation of tissue culture.
Hi Ahmed Al mayahi,
Gelling agents can affect tissue-culturing outcome. For example, in cotton transformation, we were advised to use 'PhytagelTM' as gelling agent, instead of regular agar, to alleviate browning caused by oxidation of polyphenolic substances.
1. It also depends on the types of your experiments to decide which gelling agent you should use. For example, to embed plant protoplasts in agar, high purity and low melting point (LMP) agarose/agar is usually used. Compounds in impure agar may be harmful or toxic to the protoplasts. LMP agar can avoid risk of heat-shock to the cultured protoplasts.
Researchers also found that specific type of agar can increase the viability of protoplasts embedded in it, such as Alginate (see attached paper, yellow highlights).
2. Also see this link from Sigma-aldrich. You can find out different kinds of gelling agents which can be used for plant tissue culture, including Alginic Acid.
[ http://www.sigmaaldrich.com/technical-documents/protocols/biology/gelling-agents.html ]
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2689959/
Link will be helpful
hi, the gelling agent is important for plant culture such as growth
@ Sathish Dhandapani,
Thanks for pointing out the paper. It is an interesting paper.
"Variations in the Composition of Gelling Agents Affect Morphophysiological and Molecular Responses to Deficiencies of Phosphate and Other Nutrients"
In the article, it also mentions some other studies related to this discussion:
"Studies show, however, that the agent itself causes variations in plant growth responses on otherwise identical nutrient media (Nowak and Asiedu, 1992; Scholten and Pierik, 1998a, 1998b; Beruto et al., 1999). Differences in the performance of gelling agents have been attributed to their variable physiochemical characteristics, such as nutrient diffusion rate, elemental and organic impurities, and gel strength (Debergh, 1983; Nairn et al., 1995). While the elemental contaminants in gelling agents used with nutrient-rich media may not significantly affect the growth of Arabidopsis seedlings"
As suggested by Dr Yau Phytagel is good,
But the problem is its availability.
The gelling agabts are many and the researchers has to to be choosy dpending on his spces he works and the typr of experiment he carries our. For instance Gelrite is oftwn used for sythetic seeds form somatic embryos.
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