Does someone work with the polyacrylamide gel at Licor? I would like to improve the 800 fluorescent signal, any suggestions?

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Fouad A Zouein

Karine, it might be the primary antibody. if your secondary is showing a good signal with you loading control (ie GAPDH or Actin,) its definetely the primary. i would suggest increasing your primary probing concentration. Best FZ

Karine Kettener

Hi everybody, The concentration that I am diluting my primer is 10uM, I add 0.18uM/sample. I am trying to know where we order the primers with the technique that usually do that here.

Tks a lot for the help, I really appreciate!!!

Howdy everybody, I redilute my 800 primer more concentrate and it is working very well!!! tks a lot for your help!!!