Sometimes, the message "Gel Leak" appears in the second run with the same polyacrylamide gel, but nothing is leaking.
The system pause when give the message. I will try to check again the apparatus but I noticed that in the second run, appear condensed buffer near the camera, maybe that is the problem. Tks for your help!
My bands are appearing, but very weak and I always saturated the image to see strong bands.
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