Hi,

I do not have any experience with in vitro aorta culture, but my colleague Lars Schiffmann

Schiffmann, L.M., Werthenbach, J.P., Heintges-Kleinhofer, F. et al. Mitochondrial respiration controls neoangiogenesis during wound healing and tumour growth. Nat Commun 11, 3653 (2020). https://doi.org/10.1038/s41467-020-17472-2

I think the protocol, which was used there, is adapted from this original publication:

Baker, M., Robinson, S., Lechertier, T. et al. Use of the mouse aortic ring assay to study angiogenesis. Nat Protoc 7, 89–104 (2012). https://doi.org/10.1038/nprot.2011.435

Kind regards,

Alexander Gluschko

Thank you so much

see these papers:

Article Kallikrein‐1 Blockade Inhibits Aortic Expansion in a Mouse M...

Article Urocortin 2 is associated with abdominal aortic aneurysm and...

https://pubmed.ncbi.nlm.nih.gov/18931513/

Article Association of Osteoprotegerin With Human Abdominal Aortic A...

I do not have any experience with in vitro aorta culture.

This is a wonderful paper, also feel free to ask if you need any further information.

https://www.nature.com/articles/nprot.2011.435

Thank you so much, and I also found one article and shared it here.@Houman Alimoradi