We are working in our lab about melanoma. In each in vivo experiment, we digest tumors to have a single cell suspension. The problem is that the precntage of viable cells is very low, between 10 and 20%. We are looking for another protocol through which we can get a higher percentage of viable cells.
By using the following cell isolation protocol, even if specified for glioblastoma cells in the protocol, we obained a high yield in viable tumor cells from tumors of various entities (Patient derived, PDX and mouse tumors). We've never tried melanoma, though.
Article Isolation and Culture of Primary Glioblastoma Cells from Hum...
Otherwise, you might try the dissociation procedure published in the following article, just skipping the subsequent steps for the MACS sorting:
Article Patient Derived Cell Culture and Isolation of CD133+ Putativ...
https://www.researchgate.net/post/Does_anyone_have_a_good_protocol_to_digest_solid_tumor_other_than_using_collagenase_D_and_get_individualized_tumor_cells_to_read_in_flow_cytometry
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