If radius of rotor is 5 cms while the other one is of 10 cms. Both are employed to separate equal amount of same mixture at same temperature and time. Would it require same RCF or different RCF?
From this site info [1]:
G-force can be different if Rotor Radius is different and RPM is the same. How to Calculate G-Force:
The Force exerted on a sample in a centrifuge is a function of the rotation speed of the centrifuge (RPM) and the radius of the rotor. 👇👇👇
If you know the RPM, the equations to calculate G-Force are:
▶▶ In Centimeters: RCF or G-Force = 0.00001118 x Rotor Radius x (RPM)²
OR
▶▶ In Inches: RCF or G-Force = 0.0000284 x Rotor Radius x (RPM)²
[1] https://druckerdiagnostics.com/g-force-calculator/#:~:text=The%20Rotor%20Radius%20is%20the,Rotor%20Radius%20is%2012.7%20cm.
Yes! that is the main determinant of the g force.
There are a lot of online calculators for rpm to g, usually you can select your rotor from a menu and plug in the g you want to get the rpm to set. Or you can look up the rotor radius for this calculation in the user manual.
https://www.sciencegateway.org/tools/rotor.htm
http://geneinfinity.org/sp/sp_rotor.html
Be sure to not exceed the max speed for the rotor or the centrifuge. Usually the max for a larger rotor is lower than the max speed for then centrifuge. Otherwise you can break the gyro and the rotor will come loose and ruin the centrifuge.
Yes, RCF is proportional to the radius of the rotor and to the square of RPM. Due to this square dependence of RCF on RPM, centrifugation speeds of 1000 RPM for 5 minutes and 500 RPM for 10 minutes are not interchangeable.
More on this link: https://bitesizebio.com/47991/centrifuges-centrifugation/
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