I am looking into selecting T cells with microbeads for isolation and subsequent adoptive transfer in immunodeficient NRG mice. I am hoping to do positive selection with microbeads. After column selection, I am wondering how the T cells will act in vivo after transfer. Do antibody/microbead-bound T cells act differently or are eliminated in immunodeficient NRG mice?
Hi Alex :) that generally depends on a few factors: Mostly, which target is used for the positive selection? Sometimes, binding to these surface molecules can already induce effects (e.g. activation) of the cells. Additionally, please be aware that the surface molecule might be inaccessible in downstream applications. For this reasons I would generally recommend to do negative selection - if available. For the effects specific to NRG mice I can't give any advise since I'm not familiar enough with that model, sorry. Hope this still helps :) Best, Felix
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