Polycaprolactone is present in the solution containing a protein. Will it affect the secondary structure determination using CD spectrum? Protein dissolved in a solution containing polyethylene polycaprolactone block copolymer.
This depends on the CD signal of the polycaprolactone allone. If it shows no signal, you are fine. If it has a slight background signal you can subtract it as buffer correction and the secondary content can be fitted as well. If the signal is stronger then the protein signal due to its higher concentration correction is not reliable.
Good luck,
Marco
It might affect the CD spectrum if Polycaprolactone has a secondary structure itself. Try to understand if it has CD signal. Also it might affect the quality of the CD signal (low signal/noise ratio). It has to be checked experimentally.
Finally it might have an effect on the protein secondary structure if the polymer and the protein interact.
It might have affect the secondary structure of the protein and consequently the CD signal of it. As Marco said you can substract it's probable signal as abuffer correction and hope it doesn't change the protein sec structure. Anyway, I don't think this biodegradable linear polymer would affect the sec structure of the protein if its concentration is not that high.
PECE gave almost a straight positive line up to 195 nm. We used it as the baseline for the protein solution measurement. Thanks...
Nice to hear and good luck with you CD-analysis. Now you can go forward to measure the CD with different PECE concentrations and see if the protein change its secondary structure.
Marco
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