hi, I recently worked with mycobacterium and saw a lot of paper that uses PBST to resuspend the mycobacterium pallet after centrifuge to prevent mycobacterium from clumping.
but tween 80 seems like cannot be autoclaved, Thus I want to ask that if I let PBST pass through a 0.22um filter, can it be called sterile PBST that can be used to resuspend my mycobacterium pellet?
Hey Yong seng Low,
Yes, from my experience, PBST that's filtered with a 0.22µm PVDF filter is considered sterile and can be used to resuspend your bacterial pellet. Please use autoclaved glassware and sterile syringes to do it, carry out the filtration process in a sterile hood if you want to be very sure. I hope this helps!
Most companies use 0.1µm filter to filter sterilise their PBST, if you have any of those lying around in your lab, you could use those instead.
Please refer to the product information of Tween-80 in the following:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/154/550/p6224pis.pdf
Tween-80 is not recommended to autoclave. Recommend sterilization from Sigma-Aldrich is filtration by 0.22 um filter
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