Hello,

I am growing HaCaT cell in low Ca DMEM (0.03mm) then switch to high Ca DMEM (2.8mM) to induce differentiation. I need to do a RNAi knockdown where I use Opti-MEM™ I Reduced Serum Medium.

I add 500ul of optimem + 1.5ul of oligos + 5ul of lipofectamine RNAiMAX + 500ul of my cell resuspended in low Ca DMEM.

So I need my cells to be in very low Ca to prevent differentiation.

Can anyone tell me if there is a Ca in Optimem? if so, what is the concentration?

Thanks