How can I remove AlF3 from the Colloidal solution?

Its a colloidal solution of nano materials and AlF3 forms during the reaction and its still exist in the sample even after water washings.

22 July 2020 4,889 0 View

In EFA, if the items do not get loaded under meaningful factors, what should be done?

Say item number 1, 2, and 3 are being loaded under factor A and it seems theoretically meaningful. But another item i.e. item number 4 is also being loaded under factor A and this time it doesn't...

07 May 2020 5,794 11 View

How to convert numerical data into categorical data(Class) ?

Objective : Classification of wheat & Mustard genotypes using support vector Machine (SVM) ? Since SVM can classify on the basis of categorical data(Class) only like low, medium, high; but we...

19 February 2020 6,458 3 View

Which criteria we should use to classify Barley varieties into Resistant to susceptible in case of spot blotch double digit scored data ?

can we use AUDPC value if not why ? or Can we use AUDPC % days = (AUDPC/Days to maturity)*100 Or final double digit scored data if yes then what may the scale ?

30 July 2019 6,346 2 View

Can we clone 1.3 kb insert with end blunt end and other sticky end-- ligation - vector with one end sticky and other blunt end?

I have an insert and vector with one sticky end and other blunt end, It did work when insert size was small but at the time of large size fragment it didn't. Can anybody provide me solution?

25 July 2016 2,466 5 View

How can we define the most suitable herbal seed extraction (Methanolic, water, etc) ?

01 June 2015 2,603 5 View

Does fused chromosome show increased telomere length?

If chromosomes get fused at telomere after shelthrin dysfunction, will this condition show increased telomere length in qPCR method?

07 April 2015 3,385 4 View

Is HPLC an ideal option to know the components of aqueous plant extract?

07 January 2015 7,359 12 View

How to identify the plant components?

I have prepared water extract of leaves of the plant. I have done the phytochemical analysis of that extract also. Now, I want to know what all the components are present in the extract. Can...

23 May 2014 3,164 3 View

How can I make a nutritional animal model for obesity and atherosclerosis?

I would like to work on atherosclerosis and obesity. I need an animal model for this work.

23 April 2014 410 12 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...

18 February 2021 8,719 1 View

Droplet PCR in plastic chips always result an unexpected band, any suggestion of possible contaminations and how to improve?

I am doing droplet based digital PCR inside plastic chips. I can get the correct band, but I also consistently get an unexpected band, slightly shorter. I attached a typical gel image here....

18 February 2021 6,618 2 View