Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

How structure of monomers in molecular imprinted polymers influence their selectivity?

Hello, I'm biologist and currently working on MIP. I wanted to know is there any literature that mention the influence of monomer structure (for example size of molecule) on MIP selectivity and...

27 October 2020 1,571 6 View

What mask can be used for ultra-deep KOH etching on 110 Si?

Hello everyone! I need to etch Si to the depth of 1mm approximately, not affecting the surface of the sample (which is atomically flat and must be kept as it is, so we can't anneal the sample)....

16 August 2020 9,727 1 View

Are there any papers where advantages and disadvantages of different drug delivery systems are observed?

I'm writing PhD thesis and need to compare different drug delivery systems applied to SOD delivery.

22 July 2020 7,210 2 View

Which molecular structures are stained by trypan blue and ink-vinegar for visualization of mycorrhiza?

What do these substances stain exactly?

20 June 2020 8,653 1 View

How to calculate the band structure for a two-dimensional triangular lattice?

You can provide links with calculation and clear examples of the methods used. I would also like to understand how to take into account a specific potential. How to determine the forbidden zone...

09 June 2020 9,728 2 View

Can anyone advice empirical papers on ideas and their impact on institutional change?

Does anyone know any good papers on the impact of the political elite's ideas on institutional transformations in terms of comparative politics? It will be really great if there are both...

27 February 2020 3,665 3 View

Could someone recommend a journal similar to Biodiversity Data Journal?

I'd like to publish some ideas about taxonomical database. This is possible to do in Biodiversity Data Journal or PeerJ, but I need to pay for an article. Does anybody know similar journals...

23 February 2020 6,876 3 View

Is "green energy" really "green"?

Is "green energy" really "green"? Is Green Energy Really Eco-Friendly? Isn't solar panel manufacturing too dirty? Is it not too damaging to put wind turbines in farmland? Don't we pay too much...

24 January 2020 4,067 14 View

Is it possible to purify the protein using Ammonium Sulfate precipitation only?

Dear All, I am about to start purification of many variants of not tagged recombinant protein expressed in E.coli. I need it to be at least 70% pure. Column purification would be very time...

09 January 2020 7,116 4 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How to quantify carbon deposition on CuZnAl catalyst?

Dear All, I want to quantify carbon deposition on the surface of the CuZnAl catalyst. I have tried thermogravimetric analysis, but I've got weight gain as the active Cu- get oxidised. Does anyone...

01 March 2021 1,907 3 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Where can I find public datasets with Covid-19 patient information?

Hi, I'm looking for public datasets that have CT, X-ray information, or medical records for patients with Covid-19. Does anyone have suggestions?

28 February 2021 4,760 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View