Does anybody know about any described, not inferred, phosphorylation site at Munc13 proteins? If it is the case, could you please provide the reference?
Thank you.
Dear Researchers, Is it feasible to distinguish between alpha and alpha prime martensite in the Ti6Al4V alloy under rapid solidification in the as-cast state using XRD, in addition to...
30 July 2024 3,849 0 View
through origin software
17 July 2024 4,991 0 View
I am trying to configure the instrument in Profex but I can't get a chi-squared value below 9.65 for LaB6. This is the configuration of the equipment, which is a Bruker D8 Advance with a Linxeye...
14 July 2024 4,767 1 View
I am looking for a CRISPR protocol for application in leishmania strains
08 July 2024 9,732 1 View
Simular una topología de inversor para aplicaciones de vehículos eléctricos en el software PSIM
20 June 2024 2,490 0 View
Hello, do you know what the phospholipids of the membrane of rat smooth muscle aortas are composed of? outer membrane-inner membrane
19 June 2024 221 0 View
As part of my thesis I'll be conducting a single-sample pre-post intervention analysis, in which my only DV is ordinal (7-point Likert scale). In my research proposal I said that I would use...
05 June 2024 3,122 2 View
Snowball metrics is used by scival.com to report some performance indicators. This metrics seems rather obscure from the mathematical standpoint. Unfortunately there is apparently (after a quick...
05 June 2024 7,463 0 View
Tree growth is an important aspect of forestry and forest ecology. Typically a growth rate is calculated as the difference between two stem diameter measurements over a given time interval: (dbh2...
15 May 2024 8,739 2 View
Hello, ResearchGate community, I'm currently working on a research project delving into the relationship between chronic stress and social cognition. As part of my study, I require the Tier...
14 May 2024 5,860 3 View
The rate of glucose consumption by the neocortex is reduced by over 80% during anesthesia (Sibson et al. 1998), which disables the synapses (Richards 2002) that are inundated by glial tissue (Engl...
08 August 2024 3,118 0 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
What is the cycling of matter through ecosystems and how energy drives the cycling of matter in photosynthesis and cellular respiration?
02 August 2024 9,518 2 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View