Dear Ratan Singh, could you please be more specific? Which phytorremediation technique are you interested in? I guess as it exists several techiniques depending on the type of substrate you are taking care, it may be envolved different kinds of enzymes. Or even a different one could be target of interest.
I would like to know the source of contamination and pollutants of interest. Other conditions in your design may be needed to resond more appropriately.