In-house ELISA too much signal. Any suggestions ?

09 November 2020 5,509 5 View

Hi everyone, I am considering to buy pH3000 research grade pH sensor and electrode from Sensorex. Has anybody used that before?

If not, any recs for a good pH sensor to use in the lab for in vitro assays? Thank you so much

20 September 2020 7,416 1 View

Is this journal a serious journal?

Hello everyone, I want to know your opinion about: "journal of musculoskeletal and neuronal interactions" is it a serious and real journal (not a predatory one) to publish in? Thanks

25 July 2020 3,806 4 View

Is GAPDH a suitable reference gene for qPCR in transfected cells?

I transfected Huh 7.5 cell lines with vector pDG461which harbors Cas9. I performed qPCR to make sure that Cas9 is expressed in my cell lines. I used GAPDH as a reference gene in qPCR and my...

11 July 2020 7,139 11 View

How does noise affect the detection of peak in a signal?

Imagine I have an ideal signal, V, and a noise, W, so that my observed signal is S = V + W. I observe a peak in the range of my measurement, which is associated with a peak in the ideal signal,...

24 April 2020 4,815 3 View

Why the stresses in usdfld subroutine are zero?

Hello I want to model thermal expansion in abaqus and I wrote usdfld before uexpan subroutine to get the stresses that I want to use in uexpan. The simulation is completely run with subroutine...

02 February 2020 8,445 3 View

How to calculate max principal stress in abaqus user subroutine?

Hello. I want to model expansion in abaqus and i use uexpan subroutine to model it. In subroutine i want to calculate expansions in three principal directions (EXPAN(1),EXPAN(2),EXPAN(3)), but i...

06 January 2020 5,285 4 View

Abaqus error: Too many attempts made for this increment?

Hello. I tried many ways to fix this problem such increasing increments, changing mesh size and boundary conditions,...but Abaqus still show this error. When i define static behaviour without...

09 September 2019 386 3 View

What is the best solvent/method to be use to prepare PVB (Polyvinyl butyral) solution?

I would like to spin-coat a thin film of PVB - the PVB is in powder form - which solvent should I use to prepare the PVB solution and what are the process to be followed? Thanks

04 February 2019 8,677 15 View

Could anyone tell me how to find refractive index of SiO2 thin film, from its UV/Vis transmission spectrum?

I have recorded UV/Vis transmission spectrum of SiO2 thin film deposited on glass. I want to find the refractive index of the film. My transmission spectrum does not represent interference...

01 June 2018 5,500 5 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View