Does cDNA library size have any effect on sequencing or downstream differential gene expression analysis?

03 November 2021 1 5K Report

I have prepared cDNA libraries. Library size is large in a few of my samples (680-777 bp, including adapters), does large library size have any effect on RNAseq (NovaSeq) or downstream analysis? I plan to do differential gene expression analysis.

Soner Öner-Sieben

Hi Mousumi,

size shouldn't be an issue.

Best

Soner

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