When u extract histamine in fish and analyse by UPLC, u find lot of orher peaks other than histamine. Could someone list lhe other metabolites and is there a way of identifying it.
before derivatization you can pass the sample extraction through the suitable resin and take only the histamine content, we solve this as that
Do u have some suggestion that which resin could elute only histamine and any procedure or publications ?
Dear Dr. Ahamad,
Possible metabolites are n-methylhistamine and imidazoleacetic acid. You would need a high resolution mass spectrometer to do some untargeted analyses and search the online database for known compounds.
Here is a link to a publication where they use SPE (Oasis MCX) to extract histamine from fish extract: http://www.ncbi.nlm.nih.gov/pubmed/18822416.
Good luck,
Martijn
Thanks Dr. Martijn, We do exactly the same way except, purified with Waters Oasis MCX solid-phase extraction (SPE) cartridge. Unfortunately, I don't have access to the full text.
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