i studied mechanism of resistance in sand flies in laboratory. I asked that we must separated male from female in selection before expose with insecticide.
ooohhh......so nice, There are various literature(s) dealing the DDT selection of laboratory bred sand flies. You can follow the under noted references.
Also, expecting varied content of DDT accumulation in different population of an insect specie is quit an interesting job. You can proceed for the insecticidal exposure in that way following to the separating male and female insects individually.
Good Luck.......!!!!
Does anyone used DDT for selection of sand fly in laboratory؟must we separated male from female before exposed to insecticide? - ResearchGate. Available from: https://www.researchgate.net/post/does_anyone_used_DDT_for_selection_of_sand_fly_in_laboratorymust_we_separated_male_from_female_before_exposed_to_insecticide#58b65aa693553b47db4e5524 [accessed Mar 1, 2017].
I just said that DDT was fobbiden to use in our country for long time because of its residue. Seriously speaking, seperating males and females in insecticide resistance selection is more accurante.