16 April 2014 1 5K Report

Neonatal pain is recognized to alter stress hormone levels and in turn influences neural development.

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What is the most effective way to sterilize camelina seeds?

I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...

02 August 2024 1,178 3 View

Curcumin encapsulation in Bovine Serum albumin (BSA)?

Hey all what are the ways by which an encapsulated drug inside BSA may escape from its encapsulation. We had synthesised curcumin encapsulated BSA by desolvation method followed by centrifugation...

23 June 2024 8,785 5 View

How does a Man-in-the-Middle (MitM) attack work in the context of Transport Layer Security (TLS), and what specific mechanisms can be employed ?

Context In the realm of cybersecurity, Transport Layer Security (TLS) is widely used to secure communications over a computer network. Despite its robust encryption mechanisms, TLS is still...

19 June 2024 6,595 3 View

Why is colony PCR not working ?

I've been doing colony PCR for last few days but haven't seen any bands on the gel. I've tried both positive and negative controls, but I'm still not getting any bands. Here's my procedure: I use...

07 June 2024 203 2 View

Albumin Nanoparticle Synthesis, troubleshooting?

Hey all I have tried synthesizing Albumin nanoparticles using desolvation technique using glutaraldehyde as a cross linker. After 24 hrs of stirring I could see that the protein has turned brown...

04 June 2024 1,501 0 View

If my standard quercetin equation is Y = 0.0057x + 0.0635 then how can I know the value of ethanolic extract of 10 mg/ml if absorbance value is 0.39?

I wanted to know the value of total flavonoid content in mgQE/gm. Total volume I kept in well plate is 200 µl in which extract was 20 µl. Anyone please help me with this problem

10 May 2024 5,658 4 View

What will be the value of potassium content in mg/100g if absorbance value is 45 ppm in flame photometer?

Amount of fruit powder used 0.15g; final volume of the extract was 100 ml and I have done my experiment in flame photometer. I did not know how to convert 45ppm in mg/100g. Please help me?

10 May 2024 3,173 0 View

Difficulty in Phase separation ?

Hey all I intend to isolate free circulating SiRNA in media using chloroform extraction. I have added chloroform to media containing SiRNA to get the phase separated. The Chloroform came in the...

17 April 2024 8,522 3 View

Suggestions required for use of Ion exchange resin?

For purification of carbohydrases (carbohydrate degrading enzymes) from bacterial cell free supernatant (CFS), which Ion exchange resin can be used? Are there prepacked columns available for the...

14 April 2024 3,699 0 View

Question: Herbicide formulations ?

Which is probably a better and efficient formulation for the application of Pre-emergent herbicide? A) Dry formulation B) Liquid formulation?

11 April 2024 5,157 1 View

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Which is more important: The human factor or the technological factor in combating climate change?

On a personal note, even though technology has attracted a lot of interest and funding to combat climate change, it is becoming more and more clear that taking care of the "Human Dimension" is...

02 August 2024 6,773 12 View

How can AI-driven personalized learning balance individual instruction with fostering social and collaborative skills?

This question tackles the complex issue of ensuring that while AI can tailor educational experiences to individual needs, it doesn't neglect the equally important aspect of fostering social...

02 August 2024 4,751 3 View

Recruitment for Postpartum Mental Health Research?

We are currently recruiting for two studies on postpartum mental health as part of our work at the Perinatal Mental Health Research Lab at Alliant International University. If you or someone you...

30 July 2024 2,950 0 View

Are you interested in contributing a book chapter on biomaterials in Orthopaedics?

I am editing a book for Springer Nature on recent advancements and future trends in biomaterials for orthopedic and trauma applications, with a publication date slated for early next year. If you...

29 July 2024 5,015 9 View

Can primary care be sustainable ? And how ?

I am starting to work on this question, with particular reference to what we might be able to learn from countries outside the UK. Of course this topic is huge, and I am breaking sustainability...

25 July 2024 1,545 3 View

How can we, as educators, Dismantle Deficit Ideology in Educator Professional Development?

Deficit ideology, the harmful belief that certain individuals or groups are inherently lacking in skills, knowledge, or ability, is a pervasive issue in educational settings, particularly in the...

22 July 2024 7,804 2 View

How to combat antibiotic resistance?

The increasing resistance of bacteria to antibiotics has made many infections difficult to treat, posing not only a threat to public health but also a major challenge to healthcare systems worldwide.

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The importance of humanistic care?

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