I am currently working on Mammalian and Aquatic toxicology, particularly on testing new drugs on mammalian models. I wanted to focus on new technologies and methodologies which will be future-proof. Can anyone suggest new technologies in the field of Toxicology?
I am planning at the molecular biology level including cell culture, patch clamping etc.
In general I would say that OMIC technologies become more frequently used in the field of Ecotoxicology / Toxicology, although the definition of clear endpoints with these methods is really tricky.
In particular transcriptomics are applied quite often as they tend to be less challenging compared to proteomics or metabolomics. Overal the integration of these three methods is what I think is the most challenging part right now. You can read up on these approaches here:
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Check https://www.frontiersin.org/research-topics/26773/methods-and-application-in-predictive-toxicology-2021
Also check https://www.nature.com/subjects/methods-of-toxicology-studies
Dear Lokeshkumar P. . I hope the following link might help you : https://www.resurchify.com/impact/details/25241
Alsp, consider the link : https://www.scimagojr.com/journalsearch.php?q=25241&tip=sid
PhysioMimix allows scientists to perform organ-on-chip studies in cell culture labs without using animal models. The device enables tests to be performed with new potential drugs on living human organ models. Powered by technology licensed from the Massachusetts Institute of Technology, PhysioMimix features open-well plates which allow the formation of micro-tissues that look and behave like human organs and tissues. Moreover, several devices can be linked with the help of small fluid-filled channels to analyze the interaction of organs with one another as well as their response to stimuli. PhysioMimix also has the advantage of being fast to install in cell culture labs. The open-well system allows easy introduction of primary cells, stem cells or pre-formed tissues to develop the organ mimics and remove samples for analysis during an experiment.
XCellR8’s EpiAirway model was created by using human bronchial and tracheal cells to reconstruct the airway epithelium like that of humans. It has beating cilia at the epithelial surface and can produce mucus. Whilst performing tests for inhalation toxicity, chemicals or formulations are introduced onto the EpiAirway model’s surface. To prevent excessive evaporation of volatile substances, a cap is used where required. After a three-hour exposure in a cell culture incubator, the substance under test is removed by gentle washing. Any harm that is caused to the cells is calculated by their ability to metabolize a dye (MTT) into a purple product, which is measured using a spectrophotometer. The level of toxicity is measured by calculating an ‘IC75’ value - the concentration required to minimize the viability of the cells in the EpiAirway model to 75% compared with a negative control treated with water. Items with an IC75 value below a threshold are considered as toxic to the human airway. The model has been widely used as a research tool.
You can read this
https://www.epa.gov/sites/production/files/2021-02/documents/nams_list_second_update_2-4-21_final.pdf
Good luck go on with expression profiling of specific target and markers you choose.
Regards
you can read Innovative organotypic in vitro models for safety assessment-NCBI or Future Tox II : in vitro Data & In silico Models for Predictive Toxicology- NCBI, you may also use a bioluminescent lactate dehydrogenase (LDH)- release assay(LDH-Glo Cytotoxicity Assay, Promega J2380)
Thank you Shaymaa Al- Barzanchi,
I am looking for new perspectives in the toxicology area. now I am focusing on Zebrafish for the toxicology assessment.
Thank you for your suggestion
https://www.frontiersin.org/research-topics/26773/methods-and-application-in-predictive-toxicology-2021
https://www.aiha.org/blog/explore-new-research-at-the-2021-society-of-toxicology-annual-meeting
https://www.researchgate.net/post/Does_anyone_suggest_me_new_techniques_methodologies_in_Toxicology_in_2021
Among all approaches, the use of alternative techniques replacing animals has a potential for the future research. The alternative test methods include in vitro models like cell cultures, and tissue/organ assays and in silico testing.
Hi Lokeshkumar P.,
I hope this following link will be useful for you to overcome your problem.
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