Need information about methods of treatment and limitations.
i am planning to do research on the topic " Social media as a Source of Information to Engineering Students" for this please share valuable thoughts.. which type of Hypotheses can suitable for...
27 October 2018 8,864 3 View
Dear All, I have identified a putative male sterile line in okra(Abelmoschus esculentus- through wide hybridization) (confirmed sterility with crossing/ selfing/ pollen studies). In order to...
10 October 2017 7,706 3 View
Can you suggest alternate chemical for potassium tetraborate k2b4o7 which is essential for chitinase assay?
20 December 2016 2,182 3 View
I have cloned my 3.3Kb insert having repetitive sequence in yeast expression vector, by directional cloning in E.coli. Positive Clone of 8.7Kb was confirmed by restriction analysis. While the same...
26 January 2016 4,477 2 View
electrical engineering
11 February 2015 6,989 3 View
what are the different reducing methods and reduction chemicals...
24 August 2014 2,993 3 View
I have isolated total RNA from mammalian cell culture samples. I used a Qiagen Kit. I have checked the conc and quality of RNA by Nanodrop. Ratio of 260/280 was between 2.01 and 2.09, and 260/230...
21 August 2013 4,011 16 View
I am measuring a gene which is involved in differentiation of a cell. During differentiation its level is high, compared to undifferentiated state. Upon terminal differentiation of cell, mRNA...
20 June 2013 2,588 2 View
hello friends! any one suggest me alternate pcr method for plant cloning and expression instead of using race..
25 July 2011 3,681 1 View
I have been trying to prepare root tips for chromosome counting studies in okra (Abelmoschus), but every time I am failing to get stained root tips with Feulgen stain. The root tip portion is not...
01 January 1970 5,105 4 View
I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...
23 February 2021 5,167 3 View
I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...
17 February 2021 7,029 3 View
Prosopis juliflora is considered a noxious weed but its eradication is very costly inhivitive if no impossible ! We have very strong openion that it's spread could be chacked only if it's pods are...
25 January 2021 4,433 7 View
I would like to sort from a fresh specimen of lung cancer Non-Small Lung Cancer cells and cancer-associated fibroblasts to establish two different cell cultures. Which markers should I use? Thank...
04 January 2021 7,986 3 View
Good day. I would like to extract total RNA using a kit from MRSA and candida for gene expression. i tried sub-culturing standardized inoculum (50ul) with 500ul of my plant extract sub-MIC and...
23 December 2020 5,076 3 View
Hello there, I am culturing cortical neurons from E17 mice and will need to transduce them on DIV7. Does anyone have a protocol on how to do so best? Any ideas on MOI? Im using CRISPRa plasmids,...
10 December 2020 7,900 2 View
Im trying to optimize my downstream process for AAV processing and I've been having quite a bit of trouble for some reason, I described my process below: I lysed cells in production media with...
30 November 2020 1,428 1 View
Beside dung beetles, I would like to learn more about the diversity of arthropods in silvopasture systems where livestock are kept on rotation. Also what about arthropods diversity in similar...
31 October 2020 6,632 1 View
I want to label forebrain neurons so as to trace specific (multiple) circuits. We have used AAV2/8/9 in our lab so far, and they have been pretty good. AAV-DJ seems to have more infectivity rates...
23 October 2020 9,838 2 View
Methicillin-resistant Staphylococcus aureus (MRSA) is a type of staph bacteria that has developed an immunity to antibiotics commonly used to treat staph infections. MRSA is usually not detected...
20 October 2020 8,473 8 View
