Dear Colleagues, I am looking for a way to dop of electrospining nanofibers on the HEPA filter.Someone can guide me?

Electrospining method is important for production of nanofiber .If doped with nanoparticles ,can be used for remove of contaminant such as VOC , but don't have mechanical strengh . For finding a...

24 January 2021 7,290 3 View

I have started to design a Lithium ion battery in Comsol. I need to merge the LIB model with PDEs. any idea how to do that ?

the electrode structure has secondary pore networks , so I need to use different governing equations for the electrodes. I tried to use PDEs , but I have not get any results. Does anybody have any...

02 January 2021 3,225 1 View

How many and which spin multiplicities we should choose for optimization the adsorption of O2 gas with metal complexes?

I want to optimize the interaction between the the O2 molecule and a metal nanocluster, but I need to know which multiplicities I should choose?

25 November 2020 716 2 View

Why Hiden mass spectrometer shows wrong percentages after calibration?

Hello every one I was wondering if someone could help me solve the problem that I have with my Mass Spectrometer. Actually I have already calibrated my MS device for a mixture of 60% Ar and 40 %...

19 November 2020 2,984 3 View

About nanocapsules: how can I interpret smaller nanoparticle size by enhancing the polymer amount used?

in most articles nanoparticle size decreases by lower amount of polymer

16 November 2020 4,669 6 View

Problem with converting FEM code from MATLAB into Python; what is the fastest solution?

Dear all, I developed a hybrid Phase Field-Finite Element Model (PF-FEM) for fracture of electrode particles of Li-ion batteries. [Isotropic & Hybrid] Phase Field-Finite Element Model (PF-FE......

31 October 2020 8,810 1 View

Dimensionless geometry in Ansys Fluent?

Hi Everyone. I have this dimensionless governing equations that I want to solve in Ansys Fluent. But then, in Ansys Fluent, the geometry only dimensional. So my question is, does dimensionless...

17 September 2020 9,096 3 View

What is the best method for the reaction of a acid and an epoxy monomer?

I am going to react a free fatty acid with 1,2-epoxydecane.

11 August 2020 8,227 2 View

Grow E.coli in 384 microplates without shaking?

Hello, I need to grow E. coli in 384 microplates directly from colonies. They contain an Amp resistance plasmid. We cannot shake the plate. I tested LB, but they grow poorly. Any suggestion to...

11 August 2020 5,386 3 View

How can I optimize a crystal structure?

I want to optimize a crystal structure that contains a mercury atom with six-coordinated (according to the cif file) but in one monomer of this structure only 3 atoms connected to Hg, I have...

07 July 2020 9,523 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View