Does anyone knows the easiest method to quantify calpain and calpastatin? and aevaluated their activity?

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Ru-Jeng Teng

There are several commercially available kits for the activity assay. You can simply google it. Abcam and Antibody Online have them.

Pasupathi Sundharamoorthy

I also need answer for this question?

Someone can help us.

Thanks in advance

Lara Moran

For the activity it's ok, but what about the isolation and quantification.

Try this review "Physiol Rev 83: 731–801, 2003". It contains the description about how to purify anf quantify these two proteins. I am not work on this topic but just did a few experiments for calpain activity so really can not help you with the detail.

Thank you so much, Really usefull